Practical Nuclear Magnetic Resonance Workshop
Learn to do nuclear magnetic resonance (NMR) on state-of-the-art instrumentation in the Structural Biology NMR Facility. During this workshop you will learn useful techniques and experiments on Varian Inova 500 MHz and 600 MHz spectrometers. Whether you are considering NMR as a research tool in your lab or just would like to learn more about NMR, this workshop addresses practical aspects of experimental NMR and provide valuable hands-on experience.
See our web site at:
www.umn.edu/nmr.
Dates for 2008:
January 15-17, 2008 (3-day, primary focus small molecules)
June 9-13, 2008, (5-day, primary focus biomolecules)
August TBA, 2008, (3-day, primary focus small molecules)
Minimum enrollment: 5
Maxiumum enrollment: 15
Workshop brochure
Workshop Registration
3-day workshops
Workshop Format:
Lab lecture: 9 AM-11:30 AM (10 min. break at 10 AM)
Lab (hands-on): 1 PM - 5 PM
Internal and external academic: $100/3 days (payable by CUFS #)
Industry: $300/3 days
Space in these workshops is very limited. Please reserve your space early.
Day 1:
Lab and equipment intro, VNMR software and file organization, sample
preparation, 1-D processing, phasing, referencing, weighting functions, zero-filling,
integration, plotting, simple macro
writing, sample locking, tuning, shimming, 1-D proton acquisition, 1-D
processing, 90-deg. pulse width determination.
Day 2:
1-D carbon, decoupler intro, use of a BB probe, 1-D presaturation of residual solvent, optimal
parameter set-up, 2-D COSY, 2-D NOESY, 2-D TOCSY, processing 2-D data sets.
Day 3:
1-D TOCSY, 1-D NOESY, gradients, gradient shimming, solvent suppression, 1-D wet,
indirect detection: HMQC, HSQC, HMBC.
The User's Manual will be a small bound book including topics from sample
preparation and handling, contamination sources, tube and probe options for
specific situations, selecting appropriate experiments, experimental
procedures and processing of NMR data.
Course topics may be adjusted to fit the interests of the participants who enroll.
Contact Beverly Ostrowski via email at ostro016@tc.umn.edu or phone at (612)
626-1148 with questions or to sign up for this workshop.
5-day Workshop
Workshop Format:
Lab lecture: 9 AM-11:30 AM (10 min. break at 10 AM)
Lab (hands-on): 1 PM-5 PM
Space is very limited in this workshop. Please reserve your space early.
Internal and external academic fee: $30/day where participants may select from 1-4 different days; $125 for all five days (Payable by CUFs #)
Industry Fee: $100/day or $500 for all five days
Day 1: (Beginners)
Lab and equipment intro, VNMR software and file organization, sample preparation, 1-D processing, phasing, referencing, weighting functions, zero-filling, plotting, simple macro writing, 1-D proton acquisiton, 90 deg pulse width determination.
Day 2:
1-D carbon, decoupler intro, 1-D presaturation of residual solvent, optimal parameter set-up, 2-D introduction, 2-D COSY.
Day 3:
2-D parameters and set up, NOESY, TOCSY, processing and phasing 2-D data sets, linear prediction, shaped pulses & Pbox, 1-D wet, intro to BioPack.
Day 4:
Gradients, gradient shimming, solvent suppression, indirect detection: HMQC, HSQC, HMBC.
Day 5: (Biomolecules)
Sample preparation, BioPack, and intro to 3-D NMR on labeled proteins, processing 3-D data sets, and any requested topics.
The User's Manual will be a small bound book including topics from sample
preparation and handling, contamination sources, tube and probe options for
specific situations, selecting appropriate experiments, experimental
procedures and processing of NMR data.
Topics may be adjusted to fit the interests of the participants who enroll.
The application form for this workshop can be returned via email or mail to: Beverly Ostrowski, 6-155 Jackson Hall, East Bank, Minneapolis, Minnesota 55455.
This form requires
Adobe Reader 7.0 or higher.
Contact Beverly Ostrowski via email at ostro016@tc.umn.edu or phone at (612)
626-1148 with questions.
See
pictures from the 2002 workshop,
from the
March 2003 workshop,
June 2003 workshop,
January 2004 workshop and
March 2004 workshop.
