Dr. Stephen Hecht

We are studying the metabolism and DNA interactions of carcinogens that 
are present in tobacco smoke and other environmental sources.  Our present 
work focuses on nitrosamines and aldehydes.  Nitrosamines under study are
N-nitrosopyrrolidine, N'-nitrosonornicotine, and
4-(methyl-nitrosamino)-1-(3-pyridyl)-1-butanone.  Aldehydes being
investigated include acetaldehyde and crotonaldehyde.  NMR is particularly
important for the structural characterization of nitrosamine and
aldehyde-DNA adducts.

References

Wang,M., McIntee,E.J., Cheng,G., Shi,Y., Villalta,P.W., and Hecht,S.S.
(2000) Identification of paraldol-deoxyguanosine adducts in DNA reacted with
crotonaldehyde. Chem. Res. Toxicol., 13, 1065-1074.

Hecht,S.S., Hochlater,J.B., Villalta,P.W., and Murphy,S.E. (2000)
2'-Hydroxylation of nicotine by cytochrome P450 2A6 and human liver
microsomes: formation of a lung carcinogenic precursor. Proc. Natl. Sci.,
97, 12493-12497.

Wang,M., McIntee,E.J., Cheng,G., Shi,X., Villalta,P.W., and Hecht,S.S.
(2000) Identification of DNA adducts of acetaldehyde. Chem. Res. Toxicol.,
13, 1149-1157.

Wang,M., McIntee,E.J., Cheng,G., Shi,Y., Villalta,P.W., and Hecht,S.S.
(2001) A Schiff base is a major DNA adduct of crotonaldehyde. Chem. Res.
Toxicol., 14, 423-430.

Upadhyaya,P., McIntee,E.J., and Hecht,S.S. (2001) Preparation of
pyridine-N-glucuronides of tobacco-specific nitrosamines. Chem. Res.
Toxicol., 14, 555-561.

Wang,M., McIntee,E.J., Cheng,G., Shi,Y.E., Villalta,P.W., and Hecht,S.S.
(2001) Reactions of 2,6-dimethyl-1,3-dioxane-4-ol (aldoxane) with
deoxyguanosine and DNA. Chem. Res. Toxicol., 14, 1025-1032.

Wu,M., Simpson,C.D., Christiani,D.C., Chang,L.W., and Hecht,S.S. (2001)
Relationship of exposure to coke-oven emissions and urinary metabolites of
benzo[a]pyrene and pyrene in coke-oven workers. Cancer Epidemiol. Biomarkers
& Prev., submitted.

Back